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Title: Selected biophysical and biochemical parameters of the placentas from women with pregnancy induced hypertension

Abstract:

Pregnancy induced hypertension (PIH) is a serious complication of gestation of still unknown etiopathogenesis. The data from the literature indirectly indicates that the contributing factor influencing the development of PIH is oxidative stress. This phenomenon causes the change of the amount of paramagnetic substances in the placenta. Hence the aims of the study are: 1. Assessment of the content of paramagnetic substances, inclusive of free radicals in the placenta of healthy pregnant women and women with pregnancy induced hypertension. 2. Determining the differences in the content of nitric oxide as a paramagnetic substance in the placenta of pregnant females with uncomplicated gestation and gestation complicated by PIH. 3. Determining the differences in the histological structure of the placenta of pregnant females with uncomplicated gestation and gestation complicated by PIH. Samples of the placentas collected from women with physiologically normal gestation and from those with diagnosed pregnancy induced hypertension were investigated using electron paramagnetic resonance (EPR) spectroscopy. Nitric oxide was also determined with the Griess’s method. Moreover, histopathological examination of the placenta samples was carried out, which was documented with numerous microscope photographs. The results obtained from electron paramagnetic resonance spectroscopy revealed the differences in the content of paramagnetic substances in the placentas of women with PIH as compared with the control group. Statistically significant increase in free radicals level in the placentas of the women with PIH was observed. In the spectrum of the placenta samples the presence of a second signal was found, the source of which were ions of metals from transitory groups. Its detectability is higher in the samples of the placenta from women with normal pregnancy than from those with PIH. Some ions (manganese, copper and zinc) are components of the superoxide dismutase (MnSOD and CuZnSOD, respectively), which can play a fundamental role in oxidoreductive systems. One of the paramagnetic compounds which can influence the occurrence of PIH is nitric oxide. However, EPR spectroscopy did not reveal its presence. Instead, slight amount of nitric oxide was found with the use of the indirect method – the Griess’s method. The differences in NO content between the study groups were not statistically significant. Morphological assessment of the placenta samples showed similar features in both groups, however, some morphological changes in the placentas from women with PIH were more intense. The analysis of the obtained results was the basis for the following conclusions: 1. The use of EPR spectroscopy for the determination of the paramagnetic substance content, inclusive of free radicals showed: a. Statistically significantly higher content of free radicals in the placentas from pregnancies complicated by PIH as compared to their content in the placentas from physiologically uncomplicated pregnancies. It can explain the presence of oxidative stress in the placentas of the pregnant with PIH, which was supposed to be responsible for the damage of the vascular endothelium. b. Statistically significant deficiency of ions of the metals from transitory groups in the placentas of pregnancies complicated by PIH as compared to their content in the placentas from uncomplicated pregnancies, which can have an influence on oxidoreductive systems. 2. Lack of statistically significant differences in nitric oxide content in the placentas from pregnancies complicated by PIH as compared to their content in the placentas from normal pregnancies, which may indicate the lack of the relation between the NO content and etiopathogenesis of PIH. 3. Morphological features of the placentas from pregnancies complicated by PIH are similar to those observed in the placentas from normal pregnancies. ; However, these features in the placentas from pregnancies complicated by PIH are more intense and therefore they correlate with statistically significantly higher concentration of free radicals.

Level of degree:

2 - studia doktoranckie

Degree discipline:

położnictwo ; ginekologia

Degree grantor:

Wydział Lekarski

Promoter:

Antoni Basta

Date issued:

2008

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:875

Call number:

ZB-109600

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Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego - Collegium Medicum

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Last modified:

Nov 19, 2019

In our library since:

Nov 21, 2012

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All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/875

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ZB-109600 Nov 19, 2019
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