Obiekt
Tytuł: Characteristic of group B streptococcus isolated from invasive infections and carriers
Abstrakt:
The aim of the present Ph.D. dissertation was to characterize Streptococcus agalactiae isolates (n=99). S. agalactiae (Group B streptococci; GBS) can trigger newborn infections. The phenotypic and genotypic characteristic of GBS involved marking serotypes with the use of GBS serotyping kit, and also detecting capsular polysaccharides genes and alpha-like protein genes by employing multiplex PCR reactions. The incidences of erythromycin and clindamycin resistance among GBS strains were detected. The sensitivity of GBS strains to the antagonistic activity of lactic acid bacteria strains (LAB) originating from the vagina and producing bacteriocins was tested in a mixture of fluid 24 hrs cultures. The results were determined quantitatively by serial dilutions method. An assessment of the adhesion of GBS strains to cell lines HT29 and A431 was determined. The majority of tested GBS strains belonged to serotype III and possessed alp2 gene. Most of GBS strains (n=90) were susceptible to the antibiotics used. 7 GBS strains possessed MLSB mechanism and 2 GBS isolates possessed M phenotype. Most of the investigated LAB strains had an ability to inhibit the GBS growth. L. plantarum strains were the most effective LAB strains isolated from vagina inhibiting GBS. GBS strains containing the rib gene adhered to HT29 and A431 cell lines more strongly than other GBS st ; rains. The attained information may be useful in the future to research alternative methods of eliminating vaginal GBS colonization in pregnant women.
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Data ostatniej modyfikacji:
26 cze 2023
Data dodania obiektu:
3 cze 2022
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84
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100
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http://dl.cm-uj.krakow.pl:8080/publication/4725
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| Nazwa wydania | Data |
|---|---|
| ZB-115308 | 26 cze 2023 |
Obiekty
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