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Title: Evaluation of the effect of histamine and new histamine H3 and H4 receptor ligands on the human eosinophils adhesion to endothelium

Abstract:

Histamine is an extensively studied bioorganic amine, having a broad effect on many different aspects of human physiology and pathophysiology. Histamine pleiotropic activity is a result of its ability to interact with four types of G-protein-coupled proteins, known as histamine H1, H2, H3 and H4 receptors. The aim of the presented study was to develop a new functional assay of human eosinophils adhesion to endothelium, that would allow to explore the effect of selected histamine receptors ligands on eosinophils adhesion to endothelium. For these studies, highly purified eosinophils were isolated from the human peripheral blood. Isolated cells have been tested against their viability and functionality. During the adhesion experiments histamine could in a dose-dependent manner increase the number of adherent cells to endothelium. Histamine action was exclusively dependent on histamine H4 receptor activation. The validated eosinophils adhesion to endothelium assay was further used during the pharmacological examination of the small library of histamine H3 and H4 receptors ligands. Finally, a number of preliminary studies were conducted, in order to evaluate the mechanism behind the histamine-dependent eosinophils adhesion to endothelium. In conclusion, newly developed assay proved to be a valuable new tool in pharmacological studies of histamine receptors ligands.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

farmakologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Farmaceutyczny.

Promoter:

Katarzyna Kieć-Kononowicz

Date issued:

2017

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:4392

Call number:

ZB-127775

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Oct 13, 2020

In our library since:

Oct 13, 2020

Number of object content hits:

5

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4393

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ZB-127775 Oct 13, 2020
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