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Title: T helper and T regulatory cells in systemic lupus erythematosus with kidney involvement

Abstract:

Lupus nephritis (LN) is one of the most severe manifestations of systemic lupus erythematosus. The etiology of the disease is complex and remains the subject of research.The objective of this study was to investigate the role of T CD4+ effector and T regulatory (Treg) cells in LN.34 patients with LN and 19 healthy controls were qualified for the study. Lymphocytes immunophenotyping was performed by using fluorescence-activated cell sorting to detect the chemokine receptors expression and by assessing cytokine production after stimulation with PMA and ionomycin. Treg cells were identified as CD25+CD127- and FoxP3 (transcription factor) positive. Treg cells function was defined by in vitro suppression assay, percentage of Treg cells with higher ΔE2 FoxP3 isoform expression and level of TGF-β1 secreted by stimulated in vitro Treg cells. Nineteen cytokines were measured in serum and urine by commercially available ELISA kits. mRNA expression of 43 genes form urinary cells was performed using real-time PCR.Th17 expansion in LN is not increased during disease exacerbation, but is rather related to the chronic immunosuppressive therapy. This immune signature is likely linked to the abnormal function of Treg cells, which were less suppressive in LN patients and even facilitated differentiation of Th17 cells. Type-1-cell related urine cytokines and gene markers in the urine sediment had significant diagnostic performance in detection of active LN.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

nefrologia ; immunologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Jacek Musiał ; Bogdan Jakieła

Date issued:

2019

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:4367

Call number:

ZB-131961

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Nov 16, 2020

In our library since:

Aug 6, 2020

Number of object content hits:

1

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4368

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ZB-131961 Nov 16, 2020
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