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Title: Phenotypic and molecular characterization of Enterococcus faecium strains resistant to glycopeptides isolated from epidemic outbreaks and carriers

Abstract:

The objective of the study was to examine and characterize the phenotypic and molecular properties of the E. faecium strains, resistant to glycopeptides, isolated from epidemic outbreak and carriers. Brief characteristics were presented for two groups of patients studied to assess the key risk factors affecting the incidence of VRE colonization and infection. The VREfm isolates were multidrug-resistant but all isolates were susceptible to linezolid, tigecycline, quinupristin/dalfopristin, and daptomycin. As for these properties, isolates from epidemic outbreaks and endemics almost did not differ at all. VREfm from both groups did not form biofilm in vitro on the tested substrates.The presence and the degree of expression of the assessed pathogenicity determinants were slightly diversified. The aac(6’)-Ie-aph(2”)-Ia gene was usually responsible for gentamicin resistance, and the vanB operon was behind the resistance to glycopeptides. Most VREfm had the esp, hyl and acm genes. While gelE, cylA and asa1 were found in the studied VREfm group sporadically. In PFGE, the isolates demonstrated several characteristic clones. In MLVA, most isolates from both study groups were MT282. Whereas, in MLST, most isolates tested demonstrated the presence of ST78 and belonged to the ST78 group. All isolates showed the presence of the IS16 insertion sequence. The author concluded that VREfm from epidemic outbreaks and endemics did not demonstrate major variation in their phenotypic and molecular properties. All VREfm tested had phenotypic and molecular properties that qualified them as the CC17 hyperendemic hospital meroclone.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

mikrobiologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Nauk o Zdrowiu.

Promoter:

Małgorzata Bulanda

Date issued:

2017

Identifier:

oai:dl.cm-uj.krakow.pl:4261

Call number:

ZB-128623

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Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

May 24, 2021

In our library since:

Apr 15, 2019

Number of object content hits:

1

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4262

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ZB-128623 May 24, 2021
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