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Title: Cytometric basophil activation test in diagnosis of non-steroidal anti-inflammatory drugs hypersensitivity

Abstract:

Hypersensitivity to non-steroidal anti-inflammatory drugs (NSAIDs), have become in recent years a serious diagnostic problem. Diagnostic methods routinely applied in allergological laboratories are based on provocation tests, which commonly result in the appearance of allergic reactions reproducing hypersensitivity symptoms from mild like itching, to life-threatening anaphylactic shock. The aim of this study was to determine if the Basophil Activation Test (BAT) was a useful method in the detection of non-steroidal anti-inflammatory drugs hypersensitivity. 31 patients with NSAID hypersensitivity, confirmed by a positive provocation test or a known history and 30 volunteers tolerating NSAIDs participated in the project. The BAT was performed with the test kit Flow2CAST from Bülmann. Four drugs were tested- aspirin, ibuprofen, diclofenac and metamizol in three concentrations. Cytometric examinations were performed with the use of FACS Canto flow cytometer. The results of basophil activation test were compared with results of oral provocation test with aspirin. High sensitivity (58,1%) and specificity (83,3%) of BAT evaluated for all tested drugs was observed. Optimal sensitivity and specificity of the BAT was obtained with the following drug concentrations: acetylosalicylic acid- 0,06; ibuprofen- 0,02; diclofenac- 0,06; metamizol- 0,0125 mg/ml. The sensitivity of BAT is comparable with the sensitivity of provocation tests performed in NSAIDs hypersensitive patients. The basophil activation test seem to be effective diagnostic tool in patients with NSAIDs hypersensitivity.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

alergologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Krystyna Obtułowicz

Date issued:

2014

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:4156

Call number:

ZB-126127

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

May 24, 2021

In our library since:

Mar 23, 2017

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6

Number of object content views in PDF format

6

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4156

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ZB-126127 May 24, 2021
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