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Title: Cytometric basophil activation test in diagnosis of non-steroidal anti-inflammatory drugs hypersensitivity
Abstract:
Hypersensitivity to non-steroidal anti-inflammatory drugs (NSAIDs), have become in recent years a serious diagnostic problem. Diagnostic methods routinely applied in allergological laboratories are based on provocation tests, which commonly result in the appearance of allergic reactions reproducing hypersensitivity symptoms from mild like itching, to life-threatening anaphylactic shock. The aim of this study was to determine if the Basophil Activation Test (BAT) was a useful method in the detection of non-steroidal anti-inflammatory drugs hypersensitivity. 31 patients with NSAID hypersensitivity, confirmed by a positive provocation test or a known history and 30 volunteers tolerating NSAIDs participated in the project. The BAT was performed with the test kit Flow2CAST from Bülmann. Four drugs were tested- aspirin, ibuprofen, diclofenac and metamizol in three concentrations. Cytometric examinations were performed with the use of FACS Canto flow cytometer. The results of basophil activation test were compared with results of oral provocation test with aspirin. High sensitivity (58,1%) and specificity (83,3%) of BAT evaluated for all tested drugs was observed. Optimal sensitivity and specificity of the BAT was obtained with the following drug concentrations: acetylosalicylic acid- 0,06; ibuprofen- 0,02; diclofenac- 0,06; metamizol- 0,0125 mg/ml. The sensitivity of BAT is comparabl ; e with the sensitivity of provocation tests performed in NSAIDs hypersensitive patients. The basophil activation test seem to be effective diagnostic tool in patients with NSAIDs hypersensitivity.
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Last modified:
Mar 16, 2023
In our library since:
Mar 23, 2017
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7
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http://dl.cm-uj.krakow.pl:8080/publication/4156
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| Edition name | Date |
|---|---|
| ZB-126127 | Mar 16, 2023 |
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