Object

Title: Application of LC/MS/MS technique to study metabolism of new compounds, potential drugs

Abstract:

Previous studies over two hepatoselective NO-donors revealed that only one of them, V-PYRRO/NO attenuated liver steatosis in mice, while its analog V-PROLI/NO was not effective. These studies suggest, that the explanation of distinct pharmacological effectiveness may be in different pharmacokinetic and metabolic profiles of studied compounds. Therefore, the purpose of this study was to develop and validate methods for evaluating the pharmacokinetic profile and metabolism of the V-PYRRO/NO and V-PROLI/NO. In the course of the work the method for determination of studied compounds in biological matrices, the method for evaluating the activity of cytochrome P450, and an alternative method of examining the metabolism using electrochemical oxidation were prepared. In the second part results indicated that indeed V-PYRRO/NO and V-PROLI/NO were characterized by a different pharmacokinetic profile. V-PYRRO / NO was metabolized in the liver by the cytochrome P450, which correlates with its pharmacological effect, while V-PROLI/NO was mainly excreted by the kidneys unchanged. Nonalcoholic fatty liver disease, has significantly amended the pharmacokinetic profile of both compounds, suggesting that the liver diseases can affect the pharmacokinetic profile of drugs metabolized in the liver, but also drugs excreted by other routes.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

farmakologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Farmaceutyczny.

Promoter:

Stefan Chłopicki ; Maria Walczak

Date issued:

2015

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:4040

Call number:

ZB-123721

ControlNumberVIRTUA:

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Language:

pol

Access rights:

nieograniczony

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

May 24, 2021

In our library since:

Mar 2, 2016

Number of object content hits:

442

Number of object content views in PDF format

442

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4040

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ZB-123721 May 24, 2021
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