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Title: The Analysis of susceptibility and resistance to the antimicrobial agents of Helicobacter pylori strains isolated from patients with H. pylori infection with the use of microbiological and genetic methods

Abstract:

The PhD thesis presents an analysis of prevalence ofH. pylori infection in Lesser Poland between 2010-2012, the analysis of drug resistance among isolated H. pylori strains and the results of detection of the two most prevalent point mutations conferring to clarithromycin resistance. In total, 271 patients were subjected to the study. The prevalence of H. pylori infection was 21,4%. Among patients infected with H. pylori, chronic gastritis occurred in 62,1%, peptic ulcer disease – 31% and dyspepsia in 6,9% of them. The infection of H. pylori statistically significant increased the risk of chronic gastritis and peptic ulcer disease, however, there was no significant correlation between H. pylori infection and dyspepsia. Drug resistance was tested for 58 H. pylori strains. In total, 24,1% strains were resistant to clarithromycin, 41,4% to metronidazole, 13,8% to levofloxacin and 1,7% to amoxicillin. The PCR-RFLP analysis was conducted to 79H. pylori strains (35 resistant to clarithromycin,44 susceptible) in order to detect the two most prevalent mutations in 23S rRNA gene: A2143G and A2142G conferring clarithromycin resistance. 51,4% of strains carried A2143G mutation while 34,3% - A2142G mutation. The PCR-RFLP analysis revealed 5 strains without any of the tested mutations. All clarithromycin susceptible strains were negative to both tested mutations. The evaluation of the association between the type of mutation in 23S rRNA gene and the level of clarithromycin resistance was conducted. The result showed that A2143G mutants had statistically significant lower level of resistance to clarithromycin, than A2142G mutants.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

mikrobiologia ; choroby układu trawiennego

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Farmaceutyczny.

Promoter:

Alicja Budak

Date issued:

2014

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3976

Call number:

ZB-122317

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

May 24, 2021

In our library since:

Mar 26, 2015

Number of object content hits:

17

Number of object content views in PDF format

17

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3976

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ZB-122317 May 24, 2021
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