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Title: The Analysis of susceptibility and resistance to the antimicrobial agents of Helicobacter pylori strains isolated from patients with H. pylori infection with the use of microbiological and genetic methods


The PhD thesis presents an analysis of prevalence ofH. pylori infection in Lesser Poland between 2010-2012, the analysis of drug resistance among isolated H. pylori strains and the results of detection of the two most prevalent point mutations conferring to clarithromycin resistance. In total, 271 patients were subjected to the study. The prevalence of H. pylori infection was 21,4%. Among patients infected with H. pylori, chronic gastritis occurred in 62,1%, peptic ulcer disease – 31% and dyspepsia in 6,9% of them. The infection of H. pylori statistically significant increased the risk of chronic gastritis and peptic ulcer disease, however, there was no significant correlation between H. pylori infection and dyspepsia. Drug resistance was tested for 58 H. pylori strains. In total, 24,1% strains were resistant to clarithromycin, 41,4% to metronidazole, 13,8% to levofloxacin and 1,7% to amoxicillin. The PCR-RFLP analysis was conducted to 79H. pylori strains (35 resistant to clarithromycin,44 susceptible) in order to detect the two most prevalent mutations in 23S rRNA gene: A2143G and A2142G conferring clarithromycin resistance. 51,4% of strains carried A2143G mutation while 34,3% - A2142G mutation. The PCR-RFLP analysis revealed 5 strains without any of the tested mutations. All clarithromycin susceptible strains were negative to both tested mutations. The evaluation of the assoc ; iation between the type of mutation in 23S rRNA gene and the level of clarithromycin resistance was conducted. The result showed that A2143G mutants had statistically significant lower level of resistance to clarithromycin, than A2142G mutants.

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Level of degree:

2 - studia doktoranckie

Degree discipline:

mikrobiologia ; choroby układu trawiennego

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Wydział Farmaceutyczny


Budak, Alicja ; Karczewska, Elżbieta

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tylko w bibliotece

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Last modified:

Mar 17, 2023

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Mar 26, 2015

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ZB-122317 Mar 17, 2023




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