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Piotrowska, Joanna
2014
Praca doktorska
For the quantification of peptide antibiotics (bacitracin, polymyxin B, viomycin and vancomyciny) spectrophotometric method was used. The assays were performed after condensation with dabsyl chloride, o-phthalic aldehyde and 2-nitrobenzoic aldehyde. Determinations were made by optimizing the choice of the reaction medium, temperature, time of incubation. Term of color stability was determined. Stoichiometries and reaction mechanisms were described. Methods in terms of specificity, linearity, accuracy, range and precision were tested.The use of thin-layer chromatography with densitometric detection has allowed to determine the properties of complexes of bacitracin - DBS and all of tested antibiotics with NBA. The resulting peaks are well developed and easy to interpretation. Rated durability of derivates stored at 5°C, 25°C and 37°C for 24 and 72 hours after synthesis.The usefulness of the developed methods was demonstrated by their use in the quantitative determination of the active ingredients in pharmaceutical preparations. For bacitracin derivates microbiological studies and assessment of cytotoxicity were also performed.
Kraków
2 - studia doktoranckie
farmakologia ; chemia
Wydział Farmaceutyczny
Krzek, Jan
oai:dl.cm-uj.krakow.pl:3970
ZB-122320
pol
tylko w bibliotece
Mar 10, 2023
Mar 26, 2015
17
0
http://dl.cm-uj.krakow.pl:8080/publication/3970
RDF
OAI-PMH
Ekiert, Radosław
Kępczyńska, Elżbieta
Püsküllüoglu, Mirosława
Kwiecień, Anna
Woltyńska, Hanna
Hubicka, Urszula
Pac, Agnieszka
Citation style: chicago-author-date iso690-author-date
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