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Title: Analysis of feto-maternal microchimerism on the basis of fetal genetic material isolated from maternal peripheral blood

Abstract:

Current methods of prenatal diagnosis have many limitations.The lack of the methods of effective and non-invasive diagnosis of the fetus creates the need for finding new diagnostic solutions. The discovery of fetal cells and cell-free DNA transfer to the metanal bloodstream opened up new possibilities for the analysis of the child’s genome. Fetal genetic material has become available by simple maternal blood sampling. The presence of fetal microchimerism is recognized during each pregnancy. It is a physiological phenomenon commonly found among placental mammals.This study evaluated the potential of cffDNA and mirochimeric fetal cells applications in routine prenatal diagnosis. Their qualities and prevalence have been analyzed.The experimental material consisted of 5 ml of peripheral blood from women in the second trimester of pregnancy. The study group included adult women at high risk of having a baby with a birth defect and directed by a physician for amniocentesis procedure.Microarray analysis showed significant differences in genome expression profile between maternal and fetal cells. The main study result is the development of the initial protocols for culture and identification of fetal cells from maternal blood, isolation and detection of cffDNA. Fetal sex determination test from maternal blood was developed.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

genetyka ; pediatria

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Jacek J. Pietrzyk

Date issued:

2014

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3967

Call number:

ZB-121724

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Sep 17, 2015

In our library since:

Mar 16, 2015

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4

Number of object content views in PDF format

4

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3967

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ZB-121724 Sep 17, 2015
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