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Title: Induced pluripotent stem cells derived from somatic cells and their application in treatment of osteogenesis imperfecta

Abstract:

In recent years considerable attention has been put on pluripotent stem cell research. The research has been facilitated even more by a publication in which a research group lead by Shinya Yamanaka presented possibility of reprogramming somatic cells into pluripotent state by ectopic gene expression. The generation of induced pluripotent stem cells has given new hopes on establishing convenient and potent source of cells for correction of genetic disorders and regenerative medicine. The thesis presents results of attempts to generate induced pluripotent stem cells with retroviral and lentiviral vectors. Mouse embryonic fibroblast isolation, subculturing and feeder layer preparation is also described. An outcome of non-invasive method of obtaining cell material from human donors is also depicted. After that, unsuccessful attempts of somatic cell reprogramming with lentiviral vectors are presented and discussed. Results of reprogramming of human fibroblasts and keratinocytes with retroviral vectors are also presented. Furthermore, a comparison of viral vector suitability for reprogramming of somatic cells is shown. Finally, a perspective of harnessing the obtained pluripotent stem cells in creating an in vitro gene therapy model for inborn brittle bone disease – osteogenesis imperfecta is discussed.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

transplantologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Marcin Majka

Date issued:

2013

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3907

Call number:

ZB-120650

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Jun 26, 2019

In our library since:

May 29, 2014

Number of object content hits:

42

Number of object content views in PDF format

42

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3907

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ZB-120650 Jun 26, 2019
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