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Title: Influence of rhinovirus infection on eicosanoids metabolism in asthma patients

Abstract:

Eicosanoid levels are increased in asthma and rhinovirus infections. Many asthma exacerbations are related to upper-respiratory-tract (URT) rhinovirus infection. Objective. To examine how selected eicosanoid secretion changes after HRV infection in asthmatic human bronchial epithelial cells. In the second part: to determine frequency of HRV detection in URT of patients with asthma exacerbation and to identify HRV subtypes. Methods. Evaluation of eicosanoids concentration after HRV infection was performed in primary human bronchial epithelial cells cultures. Eicosanoids (LTC4, LTD4, LTE4, PGD2, PGE2 and 5-HETE) concentration were measured with LC-MS/MS. From 55 patients with asthma exacerbations nasal aspirates were taken, RT-PCR performed. PCR products of HRV-positive samples were cloned and sequenced. Sequences were identified (BLAST) and phylogenetic tree was constructed. Results. In comparison to control HBEC, epithelia infected with HRV did not release higher amounts of analyzed eicosanoids. 5-HETE was released preferentially into baso-lateral compartment, while PGD2 and PGE2 toward apical side. Rhinoviruses were found in 49% of patients with asthma exacerbation. We classified 18 samples as HRV-A, 1 sample as HRV-B and 2 samples as HRV-C. Phylogenetic analysis of all samples revealed the presence of HRV subtype circulating in the population for over two years. Conclusion. In patients suffering from asthma, bronchial epithelium does not contribute to increased eicosanoid production in HRV infection. Polarized secretion of eicosanoids may be associated with coordinated sorting of transport proteins. There is no evidence that any specific HRV subtypes cause asthma exacerbation. Some HRV subtypes can circulate in the population over relatively long time periods causing recurrent infections in people with the same subtypes.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

pulmonologia ; immunologia ; biologia molekularna

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Marek Sanak

Date issued:

2013

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3676

Call number:

ZB-120491

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Jun 26, 2019

In our library since:

Apr 18, 2014

Number of object content hits:

20

Number of object content views in PDF format

20

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3676

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ZB-120491 Jun 26, 2019
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