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Title: Role of CXCR4 and CXCR7 receptor in development and metastasis of cervical carcinoma

Abstract:

Cervical carcinoma (CC) cells demonstrate the expression of G-protein coupled seven transmembrane domain chemokine receptors CXCR4 and CXCR7. Stromal cell-derived factor-1 (SDF-1, CXCL12) binds to CXCR4 and CXCR7, and interferon-inducible T-cell alpha chemoattractant (I-TAC, CXCL11) binds to CXCR7 receptor. Together they play important role in cancer cell biology.The goal of this dissertation was to evaluate the role of CXCR4 and CXCR7 receptor in cervical carcinoma development and metastasis in HTB-35 cell line.In this study the effects of CXCR4 and CXCR7 gene down-regulation on HTB-35 cell line growth and invasiveness were tested. The cooperation between CXCR4 and c-MET receptor was examined in cervical (HTB-35) and in rhabdomyosarcoma (RMS) cell lines (RH30). The expression level of genes involved during metastasis formation were tested. Additionally metastatic potential was performed in vivo in a murine model.The results showed that simultaneous inhibition of CXCR4 and CXCR7 activity limited proliferation of examined cells in vitro. CXCR4 receptor has a important role in growth of CC cells in vivo. Down-regulation of CXCR4 resulted in reduced primary tumor weight in murine model. Furthermore, the inhibition of SDF-1/CXCR4 axis reduced the ability of CC cell to form metastasis in lungs and spleen tissue. Similar result was observed after simultaneous down-regulation of CXCR4 and c-MET receptors what reduced growth of CC and RMS cells in vitro and in vivo. Analysis of RMS metastasis to bone marrow revealed lack of human cancer cell.This results indicate very important role of CXCR4, CXCR7 and c-MET receptors in cancer growth and metastasis potential in cervical cancer and rhabdomyosarcoma cells.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Marcin Majka

Date issued:

2013

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3651

Call number:

ZB-119633

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Jun 26, 2019

In our library since:

Jan 13, 2014

Number of object content hits:

13

Number of object content views in PDF format

13

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3651

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ZB-119633 Jun 26, 2019
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