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Title: Investigation of mechanisms of the anti-atherosclerotic effect of agmatine in apoE knockout mice

Abstract:

Agmatine is an endogenous polyamine produced by decarboxylation of L-arginine and exerts a wide array of biologic effects. It has been shown that agmatine has anti-inflammatory function, inhibits the proliferation of smooth muscle cells, protects the mitochondria and modulates fatty acid metabolism. These properties of agmatine makes it an interesting candidate for anti-atherosclerotic compound. The aim of the study was to investigate the effect of exogenous agmatine on the development of atherosclerosis in apoE-knockout mice and identify the most probable mechanism responsible for anti-atherosclerotic action of agmatine.The research was carried out on male apoE-knockout mice at the age of 8 weeks. Animals was divided into two groups: control group (on chow diet, n=10) and treatment group (on chow diet mixed with agmatine, dose 20 mg per kg of body weight per day, n=8). Both methods en face and cross section showed that agmatine by 40% inhibited formation of atherosclerotic plaques. Immunohistochemistry showed that agmatine may increase plaque stability by decreasing number of macrophages. What’s more, action of agmatine was associated with increase of the high density lipoproteins (HDL) in blood. Real-Time PCR analysis showed that modulates the oxidation of fatty acid and cholesterol biosynthesis. Widespread mitochondrial action of this drug has been demonstrated by proteomic methods as well.We are tempted to speculate that in the future, compounds with similar mechanism of action could represent new group of drugs for prevention and/or treatment of atherosclerosis and coronary heart disease.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

farmakologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Rafał Olszanecki

Date issued:

2012

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3598

Call number:

ZB-118578

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Jul 15, 2019

In our library since:

Jul 18, 2013

Number of object content hits:

9

Number of object content views in PDF format

8

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3598

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ZB-118578 Jul 15, 2019
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