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Title: Identification and characterization of melanin in choroidal melanoma by electron paramagnetic resonance spectroscopy (EPR)

Abstract:

Melanins are pigments whose physicochemical and biological properties are determined by the presence of different amounts of so-called eumelanin and pheomelanin, which arise from different melanin precursors. Each melanin contains relatively stable free radical centers that give characteristic signals easily detectable by electron paramagnetic resonance (EPR) spectroscopy. The purpose of this study is to determine the quantity and type of melanin in choroidal melanomas in correlation with patient’s clinical characteristics.Melanoma-containing samples were obtained after enucleation surgery, performed because advanced choroidal melanoma was diagnosed, not qualified to preserving eye therapy.Frozen samples were examined at liquid nitrogen temperature by EPR spectroscopy. Examination showed that choroidal melanoma cells had a low eumelanin to pheomelanin index. In most of investigated melanoma-containing samples the presence of pheomelanin was determined. Also strict correlation between patient’s clinical characteristics such as skin phototype, iris and hair color and melanin type in tumor were observed. No correlation however were found between histopathological type and quality and quantity of melanin in choroidal melanoma. Macroscopic analysing cases of choroidal melanoma were characterized by wide pigment variations. The presence of tide correlation between tumor color and type of melanin was observed. Pheomelanin fenotype occurred in tumors with low content of pigment. Tumors intensively pigmented (black, dark brown) belong to eumelanin tumors.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

okulistyka

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Bożena Romanowska-Dixon

Date issued:

2012

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3491

Call number:

ZB-117824

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Oct 29, 2019

In our library since:

Mar 21, 2013

Number of object content hits:

8

Number of object content views in PDF format

8

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3491

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ZB-117824 Oct 29, 2019
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