Object

This publication is protected and available only for logged users.
This publication is protected and available only for logged users.

Title: Analysis of the mechanisms of resistance in nonfermenting rods

Abstract:

The aims of this thesis were to study carbapenem and aminoglycoside resistance and to estimate the level of genetic relatedness among selected group of nonfermenting Gram-negative rods represented by clinical strains of Pseudomonas aeruginosa and Acinetobacter baumannii.Studies of carbapenem-resistant isolates of P. aeruginosa (50) and A. baumannii (39) revealed the presence of selected carbapenem and aminoglycoside resistance determinants among respectively 62% and 100% of strains. It was stated that carbapenem resistance among P. aeruginosa and A. baumannii were co-mediated respectively by down regulation of oprD, metalo-β-lactamases and carbapenem-hydrolysing class D β-lactamases – OXA. Whereas aminoglycoside resistance was co-mediated by enzymes that acetylate (AAC - aminoglycoside acetyltransferases) and phosphorylate (APH - aminoglycoside phosphoryltransferases) these antimicrobials. Studies of carbapenem and aminoglycoside resistance determinants revealed their simultaneous occurrence both in P. aeruginosa (30%) and A. baumannii (97,4%) strains. Analysis of genetic relatedness between carbapenem-resistant P. aeruginosa and A. baumannii isolates unveiled high level of differentiation between strains tested. The phenomenon of multiple resistance mechanisms present in P. aeruginosa and A. baumannii isolates make both species major clinical and public health concerns highlighting the need of routine testing including detection of certain determinants of resistance as well as molecular typing studies. Thorough investigations of these issues will enable to decrease the spread of these particularly dangerous pathogens.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

mikrobiologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Farmaceutyczny.

Promoter:

Alicja Budak

Date issued:

2012

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3449

Call number:

ZB-117213

ControlNumberVIRTUA:

click here to follow the link

Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Oct 28, 2019

In our library since:

Mar 7, 2013

Number of object content hits:

4

Number of object content views in PDF format

4

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3449

Show description in RDF format:

RDF

Show description in OAI-PMH format:

OAI-PMH

Edition name Date
ZB-117213 Oct 28, 2019
×

Citation

Citation style:

This page uses 'cookies'. More information