Object

Title: The potential antiatherogenic effect of doxycycline –nonselective inhibitor of matrix metalloproteinases - on experimental model of atherosclerosis – apoE-knockout mice

Abstract:

Doxycycline at subantimicrobial doses inhibits matrix metalloproteinases (MMPs) activity, and is the only MMP sinhibitor which is widely available in clinical practice. The aim of the study was to reveal whether nonspecific MMPs inhibition by tetracycline could ameliorate development of atherosclerosis in apolipoprotein E (apoE)-knockout mice. 30 female 8-week-old apoE–knockout mice (in each group n=15) on background C57BL/6J, were studied. Experimental groups received the same diet as control, mixed with doxycycline in a dose of 1.5 mg/ kg b.w./ day. Cholesterol profile was not changed by the drug. Doxycycline (1.5 mg/ kg b.w./ day) attenuated atherogenesis, measured both by “en face” method (10.25 ±1.7% vs. 15.7 ± 2.0%, p<0.05) and “cross-section” method (66 254 ± 7 468 μm2 vs. 90 687 ± 8 521 mm2, p<0.05). Insitu zymography showed marked decrease of the extent of non-specific gelatinase activity in doxycycline-treated mice (65 996 ± 11 480 mm2, p<0.001). Inflammatory indicators: sVCAM-1, MCP-1, IL-6,IL-12 as well as SAA had tendency to decrease after doxycycline, however, they did not reach statistical significance. Organ bath did not show any endothelial dysfunction in experimental mice. To our knowledge, this is the first report that shows the effect of doxycycline on atherogenesis in apoE–knockout mice.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

farmakologia ; choroby układu krążenia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Jacek Jawień

Date issued:

2011

Identifier:

oai:dl.cm-uj.krakow.pl:3426

Call number:

ZB-115826

ControlNumberVIRTUA:

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Language:

pol

Access rights:

nieograniczony

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Feb 5, 2020

In our library since:

Mar 6, 2013

Number of object content hits:

293

Number of object content views in PDF format

293

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3426

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ZB-115826 Feb 5, 2020
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