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Title: Oxidative stress parameters in diabetes 2 patients with long-termcomplications

Abstract:

Background: Diabetes mellitus is a wide spread disease with anessential impact on morbidity of contemporary societies. The quality of life and the life span of the patients depend on the occurrence of chronic diabetic complications. There are many data indicating, that oxidative stress, caused by the enhancement of the reactive oxygen species formation or impairment of the mechanisms neutralizing effects of the uncontrolled oxidation process lies behind generation and further progression of chronic diabetic complications. In diabetes, there is no evidence on the increased superoxide anion formation, as it is in inflammatory state or reperfusion of ischemic tissues. Formation of reactive oxygen species in diabetes is disseminated and is linked to a “dormant inflammatory state”, which presence is marked by an increase in basic level of C-reactive protein. Among factors linking oxidative stress with generation of chronic complications of diabetes there are: chemical action of the high glucose concentrations on cellular metabolism, protein glycation, formation of the advanced glycated end products (AGEs), activation of transcription factors and activation of protein kinase C. Search for interrelation between oxidative stress in diabetes and generation of diabetes chronic complications is still an important research challenge. Objective: The study was aim to evaluate oxidative stress parameters in patients with type 2 diabetes with and without diabetic long term complications. Materials and methods: Measurements of oxidative stress parameters have been performed in plasma, serum and hemolysate obtained from 80 type 2 diabetes patients and 80 apparently healthy, gender and age matched control subjects. The plasma total antioxidant capacity (FRAP), reduced glutathione concentration (GSH), glutathione peroxidase (GPx) and glutathione reductase (GR), γ-glutamyl transferasease (GGT) activities and uric acid concentrations were measured spectrophotometrically. C-reactive protein concentrations (hsCRP ) was determined using immunoturbidimetry. Results: FRAP, uric acid, hsCRP levels and GGT activity were significantly higher in the diabetes patients as compared with the control group. The activity of GR measured in plasma and hemolysate was higher in diabetic patients than in healthy subjects and GPx activity was significantly higher in the control group. Significant correlation between FRAP and uric acid concentrations was observed in both groups. Moreover, significant correlation between BMI, total antioxidant potential and uric acid concentration was observed. No significant correlations between oxidative stress parameters and glycemic control assessed by HbA1c, mean daily glycemia and glucose coefficient of variation were found. There were no significant differences in oxidative stress parameters between diabetes patients with and without long-term complications except of nephropathic subjects who had higher FRAP, uric acid and GR in hemolysate levels ; and lower GPx activity in plasma. Significant correlation between albuminuria, FRAP and uric acid concentration and reverse correlation between albuminuria and GPx in plasma were also observed. Conclusions: Performed studies indicate that most of the plasma antioxidant status in patients with diabetes affects the concentration of uric acid, which is either an antioxidative and prooxidative factor. Research in this study demonstrated that patients with late complications of type 2 diabetes did not show significant differences in the examined parameters as compared to patients without complications. This situation may stem from the fact that type 2 diabetes develops slowly and the occurrence of overt forms of the disease usually occurs after many years of slowly progressive disorder of glucose metabolism associated with insulin resistance, transient hiperinsulinemia and functional hipoinsulinemia. Metabolic status of patients in both groups was similar and, consequently, a balance between oxidative stress and the processes of neutralizing the effects of stress was either similar.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

biochemia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Jerzy W. Naskalski

Date issued:

2011

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3420

Call number:

ZB-115824

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Feb 5, 2020

In our library since:

Mar 6, 2013

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Number of object content views in PDF format

6

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3420

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ZB-115824 Feb 5, 2020
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