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Title: The study of L-cysteine desulfuration in mouse and human brain.Comparative studies in malignant tissues, changed by the agingprocess and cyanide poisoning

Abstract:

The objective of the investigations was to determine the direction andintensity of L-cysteine desulfuration in human brain examined postmortem in two age groups, 40–60 years and above 60 years of life, as well as in intraoperative samples of human gliomas with various malignancy grades. The aim of the paper was also to study the effect of cyanide on L-cysteine desulfuration in mouse brains. Tissue homogenates were used for determinations of rhodanese, 3-mercaptopyruvate sulfurtransferase (MPST) and γ-cystathionase, aswell as total sulfur, sulfane sulfur, reduced and oxidized glutathione, cysteine, cystine and cystathionine levels. Of the examined sulfurtransferases, the highest specific activity in mouse brain was characterized of MPST; it appears the chief enzyme in the process of cyanide detoxification. The intensity of cysteine desulfuration in human brain depends of the brain region and age. The highest specificactivity in all the regions (cerebellum, hypothalamus, thalamus, subcortical nuclei, hippocampus, frontal and parietal cortex) was seen in MPST, while cystathionase showed trace activity. The concentration ratio GSH/GSSG and Cys/CSSC, and the level of cystathionine in the first age group were significantly higher as compared to the second age group. Similarly, MPST activity (except the cerebellum) and the level of sulfane sulfur (with the exception of the hippocampus). The results suggest that with age, cystathionaseaffected cystathionine metabolism, cysteine availability and H2S production are markedly decreased what in consequence may lead toan increase of levels of homocysteine - a cause of neurodegenerative processes. In gliomas, in comparison to normal brain, the activity of sulfurtransferases was significantly diminished. The level of GSH was basically steady, although when compared to values noted in histopathologically normal human brains, the said value was approximately five times higher. The presented results have contributed to a better understanding of L-cysteine desulfuration metabolism in mammalian brains.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

biochemia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.

Promoter:

Maria Wróbel

Date issued:

2011

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3401

Call number:

ZB-115816

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Dec 11, 2019

In our library since:

Mar 5, 2013

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4

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3401

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ZB-115816 Dec 11, 2019
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