Object

Title: Clinical evaluation of DFF45 expression in ovarianendometriosis

Abstract:

Endometrium of healthy women undergoes histological and functional change, in endometriosis however no significant differences in apoptotic index of endometrial cells according to menstrual cycle were identified. Apoptosis is a multi-step process resulting in cell death and induction of two major apoptotic pathways result in activation of DFF40/DFF45 complex. DFF - DNA Fragmentation factor - complex exists in cell nucleus as a dimer composed of DFF40 and DFF45. DFF45 acts as a substrate for caspase-3 and its after cleavage active form of DFF40 is released proceeding with DNA fragmentation. The aim of the study was to evaluate DFF45 molecule as a last factor in apoptosis chain and to present DFF45 role in pathogenesis of endometriosis. 88 women who underwent standard surgery procedures were included to the study. Endometriosis was diagnosed in 26 patients and 62 women were operated for benign gynaecological problems. Pregnant women and patients with neoplastic disease were excluded from the study. Immunohistochemical analysis was performed on 50 random chosen paraffin-embedded tissues. than Western-blot analysis was performed on 127 tissue species for evaluation of the DFF45 expression. All calculations were made using Statistica v. 7.0 software and p<0.05 was considered to be statistically significant. DFF45 expression was confirmed in all tissue samples. In endometriosis expression of DFF45 was decreased compared to the eutopic endometrium in proliferative and secretory phase of menstrual cycle and the difference was statistically significant. DFF45 expression was significantly higher in normal ovarian tissue compared to ovarian endometriosis. The increased DFF45 expression was detected in the 2nd phase of menstrual cycle subsequently in endometrium and in normal ovarian tissue. No changes in DFF expression according to menstrual cycle were observed in ovarian endometriomas. Negative correlation between expression of DFF45 and stage of endometriosis (according to RCE) was confirmed. As DFF45 expression is correlated with DFF40 activity during apoptosis, decrease in DFF45 amount can be a significant factor of ectopic endometrial cells resistance to apoptosis.

Level of degree:

2 - studia doktoranckie

Degree discipline:

ginekologia

Degree grantor:

Wydział Lekarski

Promoter:

Marek Klimek

Date issued:

2007

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:1167

Call number:

ZB-106070

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Access rights:

nieograniczony

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego - Collegium Medicum

Object collections:

Last modified:

Feb 12, 2020

In our library since:

Nov 21, 2012

Number of object content hits:

469

Number of object content views in PDF format

464

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/1167

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ZB-106070 Feb 12, 2020
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