This publication is unavailable to your account. If you have more privileged account please try to use it or contact with the institution connected to this digital library.
Kwiecień, Inga
2008
Praca doktorska
The aim of the studies was to utilize differences in the activity and role of γ-glutamyl transpeptidase (γGT) and γ-cystathionase (γCT) in normal and tumor cells in order to increase safety and efficiency of anticancer therapies. It was demonstrated that cystathionine, which is a CT substrate, corrected thiol antioxidant defense in normal liver and kidney cells in EAT-bearing mice. It also protected the liver against doxorubicin toxicity without diminishing cytotoxicity of the drug for EAT cells. In addition, cystathionine corrected EAT-induced disturbances in anaerobic sulfur metabolism in the kidney (but not in the liver). γGT inhibitors (1,2,3,4-tetrahydroisoquinoline and acivicin) decreased cysteine level and disordered thiol antioxidant defense in EAT cells. In normal liver and kidney cells, they elevated cysteine level, decreased ROS and augmented sulfane sulfur biosynthesis. This means that EAT cells, in contrast to normal liver and kidney cells, are completely dependent on cysteine supply from the reaction catalyzed by γ-glutamyl transpeptidase. In HepG2 cells, GT inhibitors impaired cell vitality and increased doxorubicin cytotoxicity, which was observed in spite of the concomitant rise in GSH concentration.
Kraków
2 - studia doktoranckie
onkologia ; biochemia
Wydział Farmaceutyczny
Włodek, Lidia
oai:dl.cm-uj.krakow.pl:879
ZB-109867
pol
tylko w bibliotece
Jan 23, 2023
Nov 21, 2012
14
0
http://dl.cm-uj.krakow.pl:8080/publication/879
RDF
OAI-PMH
Czubak, Jerzy
Kowalczyk-Pachel, Danuta
Iciek, Małgorzata
Magierowski, Marcin
Citation style: chicago-author-date iso690-author-date
This page uses 'cookies'. More information I understand