Object

Title: Evaluation of bacterial microflora in the colon of children with inflammatory bowel disease

Abstract:

Inflammatory bowel diseases are chronic disorders. In the recent years research on this problem has intensified because of increasing incidence of IBD. The aim of the study was to compare numbers of bacteria in colon samples of children suffering from IBD and controls. The samples taken were: 2 biopsies collected during colonoscopy from both macroscopically normal or inflamed mucosa (normal one only in the control group) and stool. Tissue samples and stool were examined using a quantitative culture technique, a quantitative fluorescent in situ hybridization (FISH) method and denaturing gradient gel electrophoresis (DGGE). Moreover, thickness of intestine mucus layer and mucin degradation by bacterial gut flora were checked. The number of Bifidobacterium spp. was lower in all examined stool samples of children with IBD and also lower in tissue samples collected from the macroscopically inflamed mucosa as compared to controls. In case of tissue samples obtained from children with CD, the number of Streptococcus spp. was higher and in children with UC, the level of Lactobacillus spp. increased, as compared to control tissue samples. Described changes in the composition of the bacterial flora in the colon were of a qualitative character. Faecal flora in patients with IBD was richer in aerobic bacteria than in the control group. Moreover, the inflammatory process taking place in the ; intestines of children with IBD caused a thinning of the mucus layer lining the intestinal wall and the faecal flora in patients with UC the had ability do digest mucin in vitro.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

mikrobiologia ; choroby układu trawiennego

Degree grantor:

Wydział Lekarski

Promoter:

Heczko, Piotr

Date issued:

2008

Identifier:

oai:dl.cm-uj.krakow.pl:853

Call number:

ZB-108813

Language:

pol

Access rights:

nieograniczony

Object collections:

Last modified:

Aug 11, 2022

In our library since:

Nov 21, 2012

Number of object content hits:

762

Number of object content views in PDF format

3

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/853

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ZB-108813 Aug 11, 2022
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