Object

This publication is protected and can be accessed only from certain IPs.
This publication is protected and can be accessed only from certain IPs.

Title: The methylation and expression analysis of SNAI2 gene in colorectal carcinoma in comparison with staging, microsatellite instability, KRAS and BRAF mutations

Abstract:

INTRODUCTION: The SNAI2 gene encodes transcription factor Slug associated with the epithelial-mesenchymal transition and cell motility. According to molecular background, colorectal cancers can be divided into two separate entities: tumors with highly instable microsatellite DNA (MSI-H) and stable ones (MSS). Some researchers recognize low level of microsatellite instability (MSI-L) as a distinct entity. KRAS and BRAF are oncogenes in colorectal carcinogenesis. Both proteins belong to intracellular signal transduction pathway. SNAI2 gene expression is associated with the activity of this pathway. MATERIAL/METHODS: Molecular analysis was performed on 163 colorectal adenocarcinomas (SNAI2 promoter methylation, MSI status, KRAS/BRAF mutations). Immunohistochemistry with anti-Slug antibody (SLUG H-140) was performed on 116 cases. Nuclear staining has been evaluated. RESULTS: The SNAI2 gene expression in colorectal cancers is associated with metastases. The SNAI2 promoter hypomethylation is associated with the expression of this gene and correlates positively with advanced stages according to Astler-Coller and TNMLV classifications. Microsatellite instability levels are significantly different with regards to hypomethylation. The percentage of hypomethylated cancers within groups increases as follows: MSI-H, MSS and MSI-L. KRAS and BRAF mutations have no connection with the expressi ; on and the hypomethylation.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

biologia molekularna ; onkologia

Degree grantor:

Wydział Lekarski

Promoter:

Adamek, Dariusz

Date issued:

2010

Identifier:

oai:dl.cm-uj.krakow.pl:786

Call number:

ZB-111798

Language:

pol

Access rights:

tylko w bibliotece

Object collections:

Last modified:

Jun 21, 2023

In our library since:

Nov 21, 2012

Number of object content hits:

1 184

Number of object content views in PDF format

0

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/786

Show description in RDF format:

RDF

Show description in OAI-PMH format:

OAI-PMH

Edition name Date
ZB-111798 Jun 21, 2023
×

Citation

Citation style:

This page uses 'cookies'. More information