Obiekt
Tytuł: The methylation and expression analysis of SNAI2 gene in colorectal carcinoma in comparison with staging, microsatellite instability, KRAS and BRAF mutations
Abstrakt:
INTRODUCTION: The SNAI2 gene encodes transcription factor Slug associated with the epithelial-mesenchymal transition and cell motility. According to molecular background, colorectal cancers can be divided into two separate entities: tumors with highly instable microsatellite DNA (MSI-H) and stable ones (MSS). Some researchers recognize low level of microsatellite instability (MSI-L) as a distinct entity. KRAS and BRAF are oncogenes in colorectal carcinogenesis. Both proteins belong to intracellular signal transduction pathway. SNAI2 gene expression is associated with the activity of this pathway. MATERIAL/METHODS: Molecular analysis was performed on 163 colorectal adenocarcinomas (SNAI2 promoter methylation, MSI status, KRAS/BRAF mutations). Immunohistochemistry with anti-Slug antibody (SLUG H-140) was performed on 116 cases. Nuclear staining has been evaluated. RESULTS: The SNAI2 gene expression in colorectal cancers is associated with metastases. The SNAI2 promoter hypomethylation is associated with the expression of this gene and correlates positively with advanced stages according to Astler-Coller and TNMLV classifications. Microsatellite instability levels are significantly different with regards to hypomethylation. The percentage of hypomethylated cancers within groups increases as follows: MSI-H, MSS and MSI-L. KRAS and BRAF mutations have no connection with the expressi ; on and the hypomethylation.
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biologia molekularna ; onkologia
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Data ostatniej modyfikacji:
21 cze 2023
Data dodania obiektu:
21 lis 2012
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http://dl.cm-uj.krakow.pl:8080/publication/786
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| Nazwa wydania | Data |
|---|---|
| ZB-111798 | 21 cze 2023 |
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Podobne
Wójcik, Piotr
Matyja, Maciej
Kamińska, Alicja
Klimkowska, Agnieszka
Kenig, Jakub
