Phenotypic and genotypie characterization of clinical strains of Neisseria gonorrhoeae with consideration of selected virulence factors - Digital Medical Library

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Title: Phenotypic and genotypie characterization of clinical strains of Neisseria gonorrhoeae with consideration of selected virulence factors

Author:

Białecka, Joanna

Date:

Type:

Praca doktorska

Abstract:

This study presents the phenotypic characterization, with a antimicrobial susceptibility profiles, and genotypie characterization based on the NG-MAST typing for 116 strains of N gonorrhoeae. The collected strains were obtained :from women and men with uncomplicated gonorrheal infections m the Małopolska region between 2014-2021. Antimicrobial susceptibility testing was performed following the EUCAST recommendations. MIC (mg/L) were determined for penicillin, cefixime, ceftriaxone, cefotaxime, azithromycin, ciprofloxacin, tetracycline, and spectinomycin. Molecular analyses made use of the PCR method, agarose gel electrophoresis, and Sanger sequencing. Plasmid resistance of N gonorrhoeae strains (BlaTEM type Africa, American and Dutch type tetM conjugative plasmids) was identified. The ability to form biofilm and adhesion to SiHa epithelia! cell lines were evaluated. Chemiluminescence assay was employed to measure the level of respiratory burst m DMSO-differentiated HL-60 cells upon stimulation with gonococci. Differences between strains isolated :from women and men in terms of phenotypic and genotypie traits were analyzed.

Place of publishing:

Level of degree:

2 - studia doktoranckie

Degree discipline:

Degree grantor:

Rada Dyscypliny Nauki medyczne

Promoter:

Zawilińska, Barbara

Date issued:

Identifier:

oai:dl.cm-uj.krakow.pl:5137

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Language:

Access rights:

tylko w bibliotece

Subject and keywords:

N gonorrhoeae diagnostic of gonorrhea antimicrobial resistance NG-MAST molecular typing

Object collections:

Digital Medical Library > PhD thesis database

Last modified:

Jun 27, 2024

In our library since:

Jun 27, 2024

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http://dl.cm-uj.krakow.pl:8080/publication/5138

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Edition name	Date
ZB-139547	Jun 27, 2024

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