Title:

Molecular epidemiology of clinical strains Klebsiella pneumoniae, population clonal structure, virulence and antibiotic resistance

Author:

Ochońska, Dorota

Subject:

antibiotic resistance ; Klebsiella pneumoniae ; hospital ; molecular epidemiology ; virulence

Abstract:

Klebsiella pneumoniae is one of the most common agents of nosocomial infections with a severe course and high mortality rate, especially in intensive care patients. This bacterium is characterized by high adaptability, resistance to numerous antibiotics, the presence of numerous virulence factors and the ability to form biofilms on the surfaces of various biomaterials. The aim of the study was to perform a detailed characterization of clinical strains of K. pneumoniae isolated from patients hospitalized in three specialist hospitals in Poland. The laboratory tests carried out included species identification, determination of drug resistance profiles along with resistance phenotypes, detection of resistance genes, genotyping and detection of genes encoding selected virulence factors. In the created collection of 172 clinical strains of K. pneumoniae, 82.6% of isolates producing ESβL β-lactamases and 8.7% of carbapenem-resistant isolates producing KPC, VIM-1, OXA-48 carbapenemases were identified. Among the resistance genes, blaSHV was dominant, detected in 82.4% of isolates, while among virulence genes, the most common was the entB gene (91.4%). In the course of molecular typing, a hyperepidemic ST147 clone of international distribution was identified in the studied population of enterobacteria.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

mikrobiologia

Degree grantor:

Rada Dyscypliny Nauki medyczne

Promoter:

Brzychczy-Włoch, Monika

Date:

2024

Date issued:

2023

Type:

Praca doktorska

Call number:

ZB-139915

Language:

pol; eng

Access rights:

nieograniczony