Object
Title: In vitro cultures of Verbena officinalis L. as a model for research on the accumulation of bioactive compounds and as a potential, biotechnological source of these compounds
Abstract:
The research object were in vitro cultures of Verbena officinalis L. - common vervain. The herb of V. officinalis is a therapeutic raw material used in traditional medicine since a long time. The monograph on the raw material (“Verbena herb”) has been present in the European Pharmacopoeia (since the 6th issue) and in the Polish Pharmacopoeia (from its VIII issue). The herb of V. officinalis has a rich chemical composition. The latest scientific research has confirmed numerous biological properties of extracts from this raw material: i.a. antioxidative, antimicrobial anti-inflammatory, anticancer. This PhD work included biotechnological and phytochemical studies and investigation on the bioactivity of extracts from in vitro biomass and for comparative purposes from herb of a soil-grown plant. Within the study, in vitro cultures with different level of organogenesis (callus cultures and microshoot cultures) were successfully initiated. A series of tests were conducted related to the optimization of conditions for the conduct of in vitro cultures and the capacity of the obtained biomass to accumulate bioactive compounds (phenylpropanoid glycosides, phenolic acids, iridoids and flavonoids) was evaluated. The biomass of microshoot cultures as well as the herb of soil-grown plant were analyzed for the content and composition of essential oil. Within this work, comprehensive studies ; were carried out on the biological activities of the tested extracts. They included the investigation of antioxidant, antiproliferative, cytotoxicity and antibacterial activities.
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Rada Dyscypliny Nauki farmaceutyczne
Promoter:
Ekiert, Halina ; Szopa, Agnieszka
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Last modified:
Mar 22, 2024
In our library since:
Mar 2, 2023
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All available object's versions:
http://dl.cm-uj.krakow.pl:8080/publication/4964
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| Edition name | Date |
|---|---|
| ZB-136274 | Mar 22, 2024 |
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