Język metadanych
Tytuł:
The study of cerebral cortex healing process after bone marrow stromal cells transplantation
Autor:
Temat i słowa kluczowe:
bone marrow stromal cells ; cerebral cortex injury ; transplantation ; glial cells ; cell culture
Abstrakt:
Transplantation of cultured marrow stromal cells creates a new possibilities for the therapy of neurological disorders and brain injuries. The aim of this study was to assess the influence of the implantation of marrow stromal cells into the injury site of cerebral cortex regarding their effect on the healing process due to the modification of cell activity. Marrow stromal cells were harvested from Wistar rat’s femoral bone and cultured “in vitro” for 14 days. Bromodeoxiuridine (BrdU, Sigma) was added to cell culture to mark these cells. Wistar rats subjected to the mechanical brain injury /n=42/ were divided into experimental and control groups. Additional sham operated group was created, as the reference group. Animals from the experimental group were given marrow stromal cells (2x106 / 10 µl) to left cerebral cortex, just after injury. Control animals received saline alone. Animals were sacrificed after 2, 7 and 14 days. The study has shown that marrow stromal cells implantation to the injured tissue produces the change of cell activity in growing glial scare by increasing the number of macrophages, microglial cells and astrocytes. Microscopic examinations show the increased formation of glial scar and new vessels. Stromal cells are able to survive in the lesion site of brain tissue. Experiments with implantation of marrow stromal cells to the cerebral cortex present the n ; ew perspectives for the therapy of the brain injuries. Further studies are needed to assess the long-term effects of the modification of cells activity in glial scare.