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Title:
Silver nanoparticles as a tool for the study of interactions between proteins
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Subject:
silver nanoparticles ; free light chains ; amyloidosis ; protein-protein interactions ; laser light scattering
Abstract:
The aim of the study was to evaluate usefulness of silver nanoparticles (AgNPs) to study interactions between proteins. These interactions are extremely important, i.a. in the case of the development of diseases associated with the formation and deposition of protein deposits in the tissues (amyloidosis). Immunoglobulin free light chains (FLC) and anti-FLC antibodies were used in the study. In the first phase of this work basic parameters of the system and investigating methods were validated based on the antigen (FLC) - antibody model. In the second stage, the verified methodology was used to study the process of spontaneous aggregation of FLC. The usefulness of various research techniques (UV-Vis spectroscopy, TEM, DLS and the original laser light scattering method) for monitoring the aggregation process of nanoparticles was also assessed. As a result of the study it was shown that within the specified protein concentration range, the antibody-coated AgNPs aggregated in the presence of the complementary antigen. It was also reported that some FLC clones spontaneously triggered aggregation of the nanoparticles. In conclusion, AgNPs turned out to be the efficient tool for studying of protein interactions. The results obtained in this work can be the basis for further research on biological material from patients diagnosed with AL amyloidosis or other diseases associated with th ; e formation of protein deposits.
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Degree grantor:
Rada Dyscypliny Nauki medyczne