The aim of the study was to assess the anti-inflammatory and gastroprotective effects of nanoparticles and the standard form of ZnO during the simultaneous use of ketoprofen in rats. In addition, the effect of ZnONP on the pro-inflammatory proteins level in RAW 264.7 cells was determined. The results of in vivo experiments showed the advantage of using nanoparticles over the administration of ZnO in the standard form. A positive effect of ZnONP administered ip a dose of 14 mg/kg for a period of 14 days on the anti-inflammatory activity of ketoprofen was observed. The anti-inflammatory effect of ZnONP at a dose of 14 mg / kg ip in the carrageenan experiment was observed. No changes in the appearance of the gastric mucosa were observed, which indicates the protective effect of both forms of ZnO on the gastric mucosa during simultaneous administration of ketoprofen. In the case of administration of ZnO in the standard form at a dose of 14 mg/kg po and ZnONP, a statistically significant reduction of magnesium concentration in the serum of animals was observed. In vitro studies have shown a decrease in the level of pro-inflammatory proteins COX-2, cPGES and the FP receptor after ZnONP supplementation at 100 and 500 nmol, which may indicate the anti-inflammatory activity of ZnONP. The results of the above experiments allow us to assume that a better absorption of the nanoparticle form of ZnO may be responsible for a beneficial therapeutic effect. Better permeability through biological membranes of ZnONP in the case of ip may result in a stronger synergistic effect of ZnONP and ketoprofen and a stronger anti-inflammatory effect of ZnONP than the ZnO.
Apr 16, 2021
Apr 16, 2021
|Olbert, Magdalena, 2019||Apr 16, 2021|