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Application of capillary electrophoresis/frontal analysis in drug-protein binding study

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Title: Application of capillary electrophoresis/frontal analysis in drug-protein binding study

Author:

Gonciarz-Dytman, Anna

Date:

Type:

Praca doktorska

Abstract:

Drug–protein binding is an important process affecting the pharmacokinetics and activity of drug, since only the free drug can pass across biological membranes and get to its site of action. In recent years, high-performance techniques have been sought to assess physicochemical properties and to study the ADME profile of compounds.The aim of this study was to develop, optimize and validate methods for separating and determining the free fraction of dexamethasone (DXM) and pravastatin (PRA) using capillary electrophoresis/frontal analysis (CE/FA) to assess their binding to plasma proteins. CE/FA has been compared with equilibrium dialysis (ED), considered as reference method. The thermodynamics of the binding process of DXM and PRA to bovine and human albumin were evaluated. CE/FA was used to study binding of compounds with endothelial activity to blood proteins, and the relationship between physicochemical properties of the tested compounds and the degree of their binding to studied proteins were evaluated.CE/FA can be used in high-throughput screening of drug-protein binding at the early stage of drug discovery. An advantage of CE/FA over ED is the ability to directly measure the concentration of free fraction of a compound in a sample, what significantly reduces costs and shortens the duration of the analysis. It can be use to evaluate the thermodynamics of binding process.

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Level of degree:

2 - studia doktoranckie

Degree discipline:

Degree grantor:

Wydział Farmaceutyczny

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Identifier:

oai:dl.cm-uj.krakow.pl:4282

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Subject and keywords:

capillary electrophoresis drug-protein binding equilibrium dialysis albumin dexamethasone

Object collections:

Digital Medical Library > PhD thesis database

Last modified:

Jun 26, 2023

In our library since:

May 7, 2019

Number of object content hits:

109

Number of object content views in PDF format

106

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4283

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Edition name	Date
ZB-128499	Jun 26, 2023

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