Advanced search ?

Object

Access Limited

This publication is protected and available only for logged users.
This publication is protected and available only for logged users.

Title: Effect of caspase inhibitor on the efficacy of selected anti-inflammatory drugs in experimental model of sepsis in mice

Author:

Pociecha, Krzysztof

Date:

2017

Type:

Praca doktorska

Abstract:

Results of clinical studies and animal experiments of recent years support the hypothesis of increased apoptosis in lymphoid tissues in sepsis. Main goal of this study was to evaluate the effectiveness of concomitant administration of selected anti-inflammatory agents (bupropion, lisofylline, prednisolone) with a nonselective caspase inhibitor (Z-VAD-FMK) in animal model of sepsis. Moreover, a pharmacokinetic study of Z-VAD-FMK was performed. The effectiveness of tested compounds was evaluated by the measurement of markers of apoptosis, necrosis, inflammation and oxidative stress. Moreover, survival tests were carried out by the Kaplan-Meier method. Concentrations of Z-VAD-FMK and its active metabolite were measured in serum and tissues by LC MS/MS method. The results of this study indicate that apoptosis, necrosis of thymocytes and expression of IL-6 in liver of mice with experimental sepsis were most effectively inhibited after concomitant administration of Z-VAD-FMK and bupropion. Moreover, co-administration of the caspase inhibitor limited a proapoptotic potential of prednisolone in thymus. Z-VAD-FMK administered intravenously to mice is extensively metabolized to its acid derivative, which may suggest that in vivo this caspase inhibitor acts by its active metabolite. Z-VAD-FMK is rapidly distributed to tissues and reaches the highest concentrations in intestines and stomach, whereas its metabolite in intestines and liver.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

farmakologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum w Krakowie. Zakład Farmakokinetyki i Farmacji Fizycznej.

Promoter:

Elżbieta Wyska

Date issued:

2017

Identifier:

oai:dl.cm-uj.krakow.pl:4272

Call number:

ZB-129251

ControlNumberVIRTUA:

click here to follow the link

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Subject and keywords:

sepsis apoptosis inflammation mice LC/MS

Object collections:

Last modified:

May 24, 2021

In our library since:

May 2, 2019

Number of object content hits:

1

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4273

Show description in RDF format:

RDF

Show description in OAI-PMH format:

OAI-PMH

Edition name Date
ZB-129251 May 24, 2021

Objects

Similar

Effect of caspase inhibitor on the efficacy of selected anti-inflammatory drugs in experimental model of sepsis in mice

Pociecha, Krzysztof

2017
Praca doktorska

Application of LC/MS/MS technique to study metabolism of new compounds, potential drugs

Kuś, Kamil

2015
Praca doktorska

Estimatation the pharmacokinetic of propentofylline in organisms of rats

Kozaczek, Ewelina

2009
Praca doktorska

Development of a panel of protein biomarkers and their application to multi-parameter assessment of vascular endothelial function

Suraj-Prażmowska, Joanna

2019
Praca doktorska

The influence of phytoestrogens on the reproductive system in male mice

Macura, Barbara

2009
Praca doktorska

Effect of calorie restriction on the development of atherosclerotic - molecular mechanisms

Kostogrys, Renata B.

2020
Praca doktorska

Procalcitonin as a diagnostic tool in vertical transmission sespis

Ferek, Justyna

2010
Praca doktorska

Assessment of the cardiac function in mice model of dilated cardiomyopathy (Tgαq*44) and atherosclerosis (apoE/LDLR-/-) by in vivo MRI

Tyrankiewicz, Urszula Teresa.

2013
Praca doktorska
More
×

Citation

Citation style:

Contact details

Address

Medical Library
Medyczna 7
30-688 Krakow

Phone

12 658 72 56
517 385 669

E-Mail

Collecting and Elaboration Department
cyfrowabm@cm-uj.krakow.pl

Visit us!

This page uses 'cookies'. More information