Title:

Allosteric modulation of serotonin 5-HT1A and 5 HT7 receptors by zinc ions

Author:

Satała, Grzegorz

Subject:

serotonin ; 5-HT7 ; allostery ; 5-HT1A ; zinc

Abstract:

The first part of the research is devoted to mechanistic insight into the nature of direct Zn interactions with 5 HT1A receptors, investigated with in vitro radioligand experiments and functional assays. The influence of Zn ions on agonist binding was measured by saturation, competition and both association and dissociation kinetic studies using [3H]8-OH-DPAT, also in the functional tests. The obtained results showed zinc non-competitive interactions toward agonist binding site of serotonin 5 HT1A receptors. The biphasic effects, involving potentiation of both agonist binding and function at sub-micromolar concentrations of zinc ions along with the inhibition of agonist affinity at sub-millimolar zinc concentrations, were observed.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

farmakologia

Degree grantor:

Wydział Farmaceutyczny

Promoter:

Bugajski, Andrzej

Date:

2017

Date issued:

2017

Type:

Praca doktorska

Call number:

ZB-127777

Language:

pol; eng

Access rights:

nieograniczony