Object

Title: Effects of nanoporous titanium oxide on adhesion, proliferation,viability and differentiation of human adipose derived stem cells

Abstract:

The ambition of current bone biomaterials research is to design implants that induce controlled, guided, successful and rapid healing. Tiatnium and its alloys are most widely used biomaterials in hard tissue surgeries. Numerous studies have proved that the osteoblastic cells are better adapted to a nanoscale environment rather to microscale niche. Nanoporous materials are in the focus of scientific queries due to their exclusive properties and very broad area of applications. One of such material is nanostructured anodic titanium oxide (ATO) with highly ordered, vertically aligned nanotubes. It is hypothesized that nanotopography of TiO2 nanotubes more closely mirror the porous structure of native bone enhancing optimal interactions for contact osteogenesis. Yet, the best nanopore size have not been fully explored and established. Stem cells are regarded as very promising and inviting tool for tissue engineering and cell-based therapies. Adipose derived stem cells (ASCs) offer many interesting opportunities for regenerative medicine. They represent many advantages over other mesenchymal progenitors (large deposits, easier, safer and less invasive accessibility, established isolation procedure, etc.) The important goal of tissue engineering is to direct stem cells differentiation into a specific, desired cell lineage. Aim: The aim of this study was to assess cell response, ; growth, viability, metabolic activity and ability to differentiate into osteoblastic lineage of human ASCs from subcutaneous fat tissue on nanoporous TiO2 (with the pore diameters of 80 and 108 nm). Methods: A three-step electrochemical anodization process in glycerol-based electrolyte with fluoride ions was performed to produce a nanoporous oxide coat on Ti surfaces. Surface characterization of the nanostructured oxide layer and cell morphology was carried out with electron scanning microscopy. Human adipose derived stem cells were isolated from subcutaneous fat tissue of healthy donors and cultured on titanium samples for 21 days. In vitro experiments to assess cell growth (BrdU incorporation test), activity (ATP monitoring and total protein concentration) and viability (LDH release test) were carried out. Cell differentiation was determined by alkaline phosphatase activity, gene expression (BGLAP, SP7, TBX3, DLX5, RUNX2, ALPL, BMP2K, COL1A1, SPP1, NOTCH1, SPARC) and alizarin red staining. Results: A nanoporous layer of TiO2 was successfully produced on Ti surfaces using a relatively simple and economic technique. The nanostructured oxide coat significantly induced the osteo-differentiation of ASCs after 21-day in vitro culture in comparison with conventional titanium. There were no powerful differences between two nanoporous (80 nm vs 108 nm) surfaces. Conclusion: Our ; results indicate that anodic nanostructured TiO2 is safe and nontoxic biomaterial. Moreover it can induce osteo-differentiation of human ASCs from abdominal subcutaneous fat tissue in vitro culture. The potential benefit from these studies would be extremely useful in the fundamental understanding of cell biology and in the application of new modifications for biomaterials and implant design. Further in vitro and in vivo test should be performed to assess ASCa- based treatment strategies and tissue regeneration potential of implants opsonized with autologous stem cells.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

biochemia

Degree grantor:

Wydział Lekarski

Promoter:

Wybrańska, Iwona ; Jaskuła, Marian Józef

Date issued:

2015

Identifier:

oai:dl.cm-uj.krakow.pl:4062

Call number:

ZB-124228

Language:

pol

Access rights:

nieograniczony

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Last modified:

Jun 2, 2022

In our library since:

Apr 13, 2016

Number of object content hits:

1 755

Number of object content views in PDF format

1

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/4062

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