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Grabowska, Agnieszka
2014
Praca doktorska
Current methods of prenatal diagnosis have many limitations.The lack of the methods of effective and non-invasive diagnosis of the fetus creates the need for finding new diagnostic solutions. The discovery of fetal cells and cell-free DNA transfer to the metanal bloodstream opened up new possibilities for the analysis of the child’s genome. Fetal genetic material has become available by simple maternal blood sampling. The presence of fetal microchimerism is recognized during each pregnancy. It is a physiological phenomenon commonly found among placental mammals.This study evaluated the potential of cffDNA and mirochimeric fetal cells applications in routine prenatal diagnosis. Their qualities and prevalence have been analyzed.The experimental material consisted of 5 ml of peripheral blood from women in the second trimester of pregnancy. The study group included adult women at high risk of having a baby with a birth defect and directed by a physician for amniocentesis procedure.Microarray analysis showed significant differences in genome expression profile between maternal and fetal cells. The main study result is the development of the initial protocols for culture and identification of fetal cells from maternal blood, isolation and detection of cffDNA. Fetal sex determination test from maternal blood was developed.
Kraków
2 - studia doktoranckie
genetyka ; pediatria
Wydział Lekarski
Pietrzyk, Jacek J.
oai:dl.cm-uj.krakow.pl:3967
ZB-121724
pol
tylko w bibliotece
15 mar 2023
16 mar 2015
7
0
http://dl.cm-uj.krakow.pl:8080/publication/3967
RDF
OAI-PMH
Stec, Małgorzata
Styl cytowania: chicago-author-date iso690-author-date
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