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Title: Optimization of the basophil activation test in the diagnosis and qualification for treatment by means of allergen-specific immunotherapy in children with respiratory allergy to house dust mites

Abstract:

Among possible alternatives for routine allergy diagnostic tests, hopeful mentions can be found of the basophil activation test (BAT), which replicates in vitro reactions that occur in the body during basophil response to allergens, thus giving a chance for forecasting the severity of allergic reaction. Aim: The aim of the study was to optimize the BAT in the detection of house dust mite allergy in Polish children with allergic respiratory diseases. Patients and methods: The study involved 32 patients (13 girls, 19 boys, median 9,5 years), with symptoms of asthma or allergic rhinitis, who were qualified for allergen-specific immunotherapy (sIT) with the allergen of the mite Dermatophagoides pteronyssinus by an experienced pediatric allergist. The control group consisted of an equal number of sex- and age-matched children with the same clinical diagnoses, yet sensitized to allergens other than D. pteronyssinus. In all patients, the BAT test was performed with D. pteronyssinus allergen. The results were analyzed by the means of Receiver Operator Characteristics to determine the optimal cut-off point.Results: The highest diagnostic efficiency in the analyzed population was yielded by the protocol assuming the cut-off of 9.76% of activated basophils after activation with a single allergen concentration of 2.25 ng/ml (sensitivity 90,63%, specificity 100%). The computed value differed significantly from the cut-off value of 15% as proposed by the manufacturer of the test. Conclusions: BAT is useful method in diagnosis and qualification of children with asthma and allergic rhinitis for the sIT with house dust mite allergens.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

pulmonologia ; immunologia

Degree grantor:

Uniwersytet Jagielloński. Collegium Medicum. Wydział Farmaceutyczny.

Promoter:

Radosław Śpiewak

Date issued:

2014

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:3917

Call number:

ZB-121453

ControlNumberVIRTUA:

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Language:

pol

Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego- Collegium Medicum

Object collections:

Last modified:

Jun 26, 2019

In our library since:

Nov 25, 2014

Number of object content hits:

8

Number of object content views in PDF format

8

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3917

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ZB-121453 Jun 26, 2019
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