Background: Experiments were aimed to test the influence of treatment with different opioids (morphine, fentanyl, methadone) on the humoral and cell-mediated immune responses. Methods: Mice were treated intraperitoneally with opioids and either immunized with sheep red blood cells (SRBC) to test the antibody production or skin-sensitized with hapten picryl chloride to induce contact hypersensitivity. The effects of opioids on the production of reactive oxygen intermediates and cytokines by peritoneal macrophages and on the expression of surface markers on these cells and blood leukocytes were estimated. Results: Opioids caused an enhancement of reactive oxygen intermediates and cytokines production when macrophages were stimulated with zymosan or LPS and reduced the expression of antigen presentation markers on macrophages. Numbers of anti-SRBC plaque forming cells and antibodies titres were lower in mice treated with all tested opioids. Effects of the opioid treatment on contact hypersensitivity were diverse. While morphine decreased the early and late phases of induction of contact hypersensitivity responses, methadone increased both reactions. In case of the effector phase of contact hypersensitivity response, morphine and fentanyl increased both its early and late stages, while methadone decreased the late reaction. Conclusions: The results of experiments show in disertation that the action of opioids on the immune system depends on type of opioid, character of response (humoral versus cellular) and types of cells involved. Here macrophages seem to play a significant role.
Uniwersytet Jagielloński. Collegium Medicum. Wydział Lekarski.
Jun 26, 2019
Nov 6, 2013
|ZB-119051||Jun 26, 2019|
Kościelniak-Merak, Barbara Karolina