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Title: Phenotypic and genotypic characterization of alertpathogens isolated in the hospital environment

Author:

Mrowiec, Paulina

Date:

2013

Type:

Praca doktorska

Abstract:

The aim of the PhD thesis was to determine the prevalence of alert pathogens among patients hospitalized between October 2008 and February 2010 in different departments of the Rydygier’s Hospital in Krakow. Moreover, the phenotypic methods (disk diffusion, Etest, the automatic system VITEK2 Compact) and genetic methods (PCR,multiplex-PCR, RT-PCR and RAPD-PCR) were applied in order to determine susceptibility patterns, the occurrence of resistance mechanisms and the genetic relatedness in the selected group of alert pathogens. Three hundred and eighty-one alert bacterial pathogens were tested including: Gram-negativerods, represented by multidrug-resistant nonfermenting strains of A. baumannii (105) and P.aeruginosa (46); strains of the Enterobacteriaceae family: K. pneumoniae (96), E. coli (38), E. cloacae(11), producing extended-spectrum betalactamases; and Gram-positive cocci represented by methicillin-resistant S. aureus (85).The monitoring of the susceptibility to antibacterial agents used in the clinical practice is necessary due to the rapid spread of the bacterial resistance mechanisms. The current epidemiological data as well as knowledge of resistance patterns of alert pathogens can provide useful information, which can be used to select the most effective treatment to avoid further rising of the resistance among microorganisms inhabiting the hospital environment.

Place of publishing:

Kraków

Level of degree:

2 - studia doktoranckie

Degree discipline:

mikrobiologia

Degree grantor:

Wydział Farmaceutyczny

Promoter:

Budak, Alicja

Date issued:

2012

Identifier:

oai:dl.cm-uj.krakow.pl:3568

Call number:

ZB-118359

Language:

pol

Access rights:

tylko w bibliotece

Subject and keywords:

alert pathogens PCR molecular typing resistance nosocomial infections

Object collections:

Last modified:

Mar 14, 2023

In our library since:

Jul 17, 2013

Number of object content hits:

8

Number of object content views in PDF format

0

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/3568

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Edition name Date
ZB-118359 Mar 14, 2023

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