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Title: Supramolecular protein ligands - conditions of binding to native proteins


Congo red and a group of structurally related dyes long used to stain amyloid proteins. In water solutions their molecules are arranged in ribbon-like micellar forms with liquid crystalline properties. Congo red could form complexes with proteins by adhesion to beta-conformation polypeptide chains. Congo red was used to check whether monocyte activation could be connected with binding of supramolecular ligands. Tight cell-cell contacts generating torsional effects may be expected to produce alteration of receptor structure, making them accessible for binding supramolecular dyes. In this study, Congo red was used to observe the binding accessibility of (I) monocytes (human) induced by contact with cancer cells (HCV29T, human), (2) macrophage (mouse line) stimulated by interaction with heat-aggregated IgG and (3) macrophage (mouse line) activated by rosetting in the presence of an SRBC-anti-SRBC system. Microscopic studies confirmed the activation of monocytes manifested by their clustering and Congo red binding, but only tightly clustered cells appeared to attach the dye on the surface. The association of amphibian (Xenopus Iaevis) egg yolk platelet proteins with Congo red, represented predominantly by lipovitellin, was studied as a model of influence the supramolecular dye binding on protein function. The native structural forms bind Congo red and produce a green birefr ; ingence effect. The gel -like nature and Congo red binding of platelets correspond to characteristics of amyloid proteins. Congo red-stained platelets seem resistant to calcium ion-driven disintegration. This effect interfered with function of the egg yolk platelets.

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2 - studia doktoranckie

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Wydział Lekarski


Konieczny, Leszek

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tylko w bibliotece

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Last modified:

Jul 7, 2022

In our library since:

Nov 21, 2012

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Edition name Date
ZB-100464 Jul 7, 2022


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