Title:

The influence of surgical operation on [gamma delta] and [alfa beta] T cell distribution in the mouse

Author:

Majcher, Paweł

Subject:

gastrectomy ; suppression ; adhesion molecules ; [gamma delta] T lymphocytes ; TGF-[beta]

Abstract:

The aim of the present study was to characterize surgery-induced changes in distribution and immune function of ab and gd T cells. Presented data shows distinct changes in distribution pattern of both T cell populations after partial gastrectomy. There was significant decrease in percentage of ab T cells in mesenteric lymph nodes, Peyer’s patches and peripheral blood on the third day after partial gastrectomy. At the same time there were no significant changes in distribution of ab T cells in peripheral lymph nodes and in the spleen. In contrast the percentage of gd T cells in mesenteric lymph nodes, Peyer’s patches and peripheral lymph nodes was significantly increased on day 3. Simultaneously the percentage of gd T cells in peripheral blood was decreased, whereas no changes were observed in the spleen. The gastrectomy-induced changes in distribution of both T cells populations were most pronounced at day 3 after the surgery, whereas they disappeared almost completely on day 7. Laparotomy alone (sham operation) had no or a little effect on T cell numbers in all tested lymph organs. Hence the severity of the trauma may be of great importance. The data obtained from in vitro experiments showed suppressor activity of gd T lymphocytes isolated from MLN of gastrectomized mice. The gastrectomy-induced regulatory gd T cells suppressed proliferation of T effector lymphocytes in vitro via released TGF-b. The regulatory T cells were not induced in mesenteric lymph nodes of sham-operated mice.

Level of degree:

2 - studia doktoranckie

Degree discipline:

chirurgia ; immunologia

Degree grantor:

Wydział Lekarski

Promoter:

Marian Szczepanik

Date:

2005

Date issued:

2005

Type:

Praca doktorska

Format:

application/pdf

Call number:

ZB-101896

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Access rights:

nieograniczony

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego - Collegium Medicum

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