Metadata language
Title:
The research of possibility bacterial translocation of Staphylococcus aureus in burn-injury patients
Author:
Subject:
attachment ; bacterial translocation ; Staphylococcus aureus ; cytokines ; transcripts ; invasion
Abstract:
Little is known about the mechanisms involved in bacterial translocation from the intestinal lumen to extraintestinal sites although it is known that Staphy/ococcus aureus can colonize the intestinal tract thereby presenting a potential resevoir for antibiotic resistant Staphylococcus aureus. The experiments described in my thesis were designed to investigate the nature of the interactions between clinicallyisolated Staphylococcus aureus strains and cultured intestinal epithelial and blood vessel endothelial cells with a view to clarifying the relative contribution of intestinal translocation of Staphylococcus aureus to nosocomical systemie infections and bacterial sepsis. Objectives of work: ► Establish a relationship between bacterial translocation of Staphylococcus aureus across epithelial and endothelial monolayers and bacterial sepsis ► lnvestigation of the mechanisms of bacterial translocation of Staphylococcus aureus using in vitro cell lines. Initially samples were obtained from burn-injury patients; samples were derived from scald, blood and intestine. Further work was focused on three parts. 1. Analyses of phenotype of S. aureus strains 2. Analyses of genotype of S. aureus. 3. In the third part the mechanism of bacteńal translocation was investigated using both culture collection strains and the selected clinical isolates The work undertaken ; in part 3 involved assessing the attachment, internalization, translocation of S. aureus. The impact of such interactions on cytokines gene expression - IL-8 i TNF-a. General conclusions: ► Bath the mutant and wild-type culture collection strains of S. aureus attached, internalized and tranclocated across intestinal epithelia! cells. ► Specific strains of clinical isolates of S. aureus intemalized efficiently within intestinal epithelia! and endothelial cells, ► Specific strains of clinical isolates of S. aureus were found not to translocate except for one strain. ► lnternalization of Staphylococcus aureus induces cytokine gene expression, it depend on time incubation and concentration of bacteria. ► lnhibition of tyrosine kinase signalling using genistein prevented S. aureus invasion.