Tytuł:

Mechanisms of negative regulation of the immune response in experimental allergic encephalomyelitis (EAE)

Autor:

Tutaj, Monika

Temat i słowa kluczowe:

tolerance ; cytokines ; experimental allergic encephalomyelitis ; multiple sclerosis ; T suppressor cells

Abstrakt:

Presented work describes a new method of skin induced tolerance via epicutaneous (e.c.) immunization. The aim of this procedure was to delay onset of EAE and reduce its severity. Employing B10.PL mice (mice that develop chronic form of EAE) it was shown that e. c. application of myelin basic protein (MBP) before induction of EAE delayed onset of EAE and reduced disease severity by 50-70% when compared to positive control. The optimal dose of e. c. applied MBP that significantly ameliorates course of a disease is between 0,1-1 mg. Skin induced tolerance is mediated by Tαβ CD4+ CD8+ double positive cells whereas Tγδ and NK-T cells are not involved in this process. Both in vivo and in vitro studies showed that skin induced suppression is antigen non-specific. Epicutaneously induced T suppressor cells (Ts) inhibit inflammatory response via released TGF- β, while IL-4, IL- 10 and IL-13 do not play a crucial role in the observed suppression.

Miejsce wydania:

Kraków

Stopień studiów:

2 - studia doktoranckie

Dyscyplina:

immunologia

Instytucja nadająca tytuł:

Wydział Lekarski

Promotor:

Szczepanik, Marian

Data:

2005

Data wydania:

2004

Typ:

Praca doktorska

Sygnatura:

ZB-100898

Język:

pol

Prawa dostępu:

nieograniczony