Presented work describes a new method of skin induced tolerance via epicutaneous (e.c.) immunization. The aim of this procedure was to delay onset of EAE and reduce its severity. Employing B10.PL mice (mice that develop chronic form of EAE) it was shown that e. c. application of myelin basic protein (MBP) before induction of EAE delayed onset of EAE and reduced disease severity by 50-70% when compared to positive control. The optimal dose of e. c. applied MBP that significantly ameliorates course of a disease is between 0,1-1 mg. Skin induced tolerance is mediated by Tαβ CD4+ CD8+ double positive cells whereas Tγδ and NK-T cells are not involved in this process. Both in vivo and in vitro studies showed that skin induced suppression is antigen non-specific. Epicutaneously induced T suppressor cells (Ts) inhibit inflammatory response via released TGF- β, while IL-4, IL- 10 and IL-13 do not play a crucial role in the observed suppression.