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Title: Secondary metabolites in in vitro cultures of Ginkgo biloba L.

Author:

Szewczyk, Agnieszka

Date:

2008

Type:

Praca doktorska

Abstract:

Ginkgo biloba callus cultures were established from male and female leaves explants. Suspension cultures were derived from callus cultures. Murashige-Skoog medium supplemented with Picloram (4 mg/l) and BAP (2 mg/l) was the best medium for further in vitro cultures cultivation. The contents of ginkgotoxin and pyridoxamine were analysed in leaves, seeds and in vitro cultures. The comparison of ginkgotoxin and pyridoxamine contents in male and female leaves collected every week in July 2005 revealed that in the middle of July the amount of ginkgotoxin is the highest.The contents of monomeric flavonoids and phenolic acids after hydrolysis of glycosides and biflavonoids were investigated by HPLC method. The results suggest that G. biloba in vitro cultures can accumulate these compounds, but only in small amounts. The phytochemical analysis of methanol extracts from callus cultures (two lines) resulted in the isolation of 12 compounds which were identified as β-sitosterol, 7-oxo-β-sitosterol, 7α-hydroxy- and 7β-hydroxy-β-sitosterol, 3-O-β-glucopyranoside-β-sitosterol, p-hydroxycinnamic alkohol, esculetin, ethyl p-hydroxybenzoate, p-hydroxybenzoic acid, caffeic acid, protocatechuic acid, 6-hydroxykynurenic acid. The occurence of these compounds in in vitro cultures of the plant was been shown for the first time.

Level of degree:

2 - studia doktoranckie

Degree discipline:

farmacja

Degree grantor:

Wydział Lekarski

Promoter:

Wanda Kisiel

Date issued:

2007

Format:

application/pdf

Identifier:

oai:dl.cm-uj.krakow.pl:1004

Call number:

ZB-107613

ControlNumberVIRTUA:

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Access rights:

tylko w bibliotece

Location of original object:

Biblioteka Medyczna Uniwersytetu Jagiellońskiego - Collegium Medicum

Subject and keywords:

in vitro cultures Ginkgo biloba secondary metabolites

Object collections:

Last modified:

Jun 27, 2019

In our library since:

Nov 21, 2012

Number of object content hits:

9

Number of object content views in PDF format

9

All available object's versions:

http://dl.cm-uj.krakow.pl:8080/publication/1004

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Edition name Date
ZB-107613 Jun 27, 2019

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