TY - GEN A1 - Kownacki, Patryk N2 - Recent studies have shown that ghrelin exhibits protective effect in the stomach and pancreas and accelerates the healing of gastric and duodenal mucosa. The aim of present study was to examine the influence of ghrelin administration on oral mucosa integrity and healing of chronic oral ulcers, as well as to evaluate the role of growth hormone and insulin-like growth factor-1 (IGF-1) in these processes. Methods: Studies were performed on three groups of animals: (1) pituitary- and salivary-intact rats; (2) sialoadenectomized rats with intact pituitary; (3) hypophysectomized rats with intact salivary glands. Chronic ulcers of the gingiva and tongue were induced by acetic acid. After induction of ulcers, rats were treated intraperitoneally twice a day with saline or ghrelin (4, 8 or 16 nmol/kg/dose) for six days. Also animals without induction of ulcers were treated with saline or ghrelin (8 nmol/kg/dose) for six days. Results: In animals with intact pituitary and salivary glands without induction of oral ulcers, treatment with ghrelin was without effect on oral mucosa morphology, mucosal blood flow or cell proliferation in oral mucosa. In rats with intact pituitary and salivary glands, administration of ghrelin, after induction of gingival and lingual ulcers, significantly increased healing rate of these ulcers. Ghrelin given at the dose of 8 or 16 nmol/kg/dose caused the stronge N2 - st and similar therapeutic effect. This result was associated by a significant increase in blood flow and cell proliferation in gingival and lingual mucosa, as well as by a significant decrease in mucosal concentration of pro-inflammatory interleukin-1β. Sialoadenectomy alone, without induction of ulcers, caused a reduction in cell proliferation and an increase in concentration of pro-inflammatory interleukin-1β in oral mucosa. In these sialoadenectomized rats, administration of ghrelin reversed the sialoadenectomy-induced reduction in cell proliferation and decreased the sialoadenectomy-induced increase in concentration of pro-inflammatory intereleukin-1β in gingival and lingual mucosa. In sialoadenectomized rats with induction of gingival and lingual ulcers, healing rate of these ulcers was reduced. As in rats without induction of ulcers, this effect was associated with a reduction in blood flow and cell proliferation in oral mucosa, as well as with an additional increase in mucosal concentration of pro-inflammatory interleukin-1β. In these rats, administration of ghrelin reversed deleterious effect of sialoadenectomy and ghrelin given at the dose of 8 or 16 nmol/kg/dose increased the healing rate of oral ulcers above a value observed in control rats with intact salivary glands. Hypophysectomy alone was without effect on morphology, cell proliferation, blood flow and concentr N2 - ation of interleukin-1β in oral mucosa. Also, in rats with induction of oral mucosa ulcers, hypophysectomy failed to affect the healing rate of these ulcers or blood flow, cell proliferation or concentration of interleukin-1β in gingival and lingual mucosa. On the other hand, administration of ghrelin was without any effect on healing rate and other parameters in oral mucosa in hypophysectomized rats. In salivary- and pituitary-intact rats, as well as in sialoadenectomized rats with intact pituitary, therapeutic effect of ghrelin in the healing of oral mucosa ulcers was well-correlated with the ghrelin-induced increase in serum concentration of growth hormone and IGF-1. Hypophysectomy increased serum concentration of endogenous ghrelin, but serum concentration of growth hormone was reduced below limit of detection, whereas IGF-1 was decreased by 90%. Administration of exogenous ghrelin failed to affect serum concentration of growth hormone or IGF-1 in hypophysectomized rats., Conclusions: Treatment with ghrelin accelerates healing of chronic ulcers gingival and lingual mucosa in rat with a primary normal oral mucosa, as well as in rats with a reduction of mucosa cell proliferation evoked by sialoadenectomy. This therapeutic effect of ghrelin is mediated by the release of endogenous growth hormone and IGF-1. Hypophysectomy abolishes stimulatory effect of ghrelin on the release o N2 - f growth hormone and IGF-1, and eliminates the therapeutic effect of ghrelin on the healing of oral mucosa ulcers. L1 - http://dl.cm-uj.krakow.pl:8080/Content/920/Patryk+Kownacki+doktorat+wersja+ostateczna.pdf CY - Kraków L2 - http://dl.cm-uj.krakow.pl:8080/Content/920 PY - 2009 KW - inflamation KW - ulceration of oral mucosa KW - interleukin KW - grelin KW - tissue blood flow T1 - Therapeutic influence of grelin on healing process of experimentally induced mucosal ulceration in the oral cavity UR - http://dl.cm-uj.krakow.pl:8080/dlibra/publication/edition/920 ER -