Search for: [Abstract = "were randomly selected and observed within 18 months after the surgical treatment. A total of 120 tissue samples was obtained during surgery. RCAS1 was estimated in 51 tissue samples derived from laryngeal and pharyngeal squamous cell carcinoma, 34 elear surgical margins, 8 nasal polyps, and 27 healthy mucous membrane specimens. Surgically removed materiał was evaluated to determine histological type and metastases of the lymph nodes using histological methods in Pathology Department of Jagiellonian University. In 27 cases of laryngeal and pharyngeal cancer two samples were collected, one derived from the cancer tissue and one from its elear surgical margin. The elear surgical margin was defined as the lcm2 area of tumor adjacent tissue macroscopically and histological free of neoplasmatfo texture. Directly after the surgical procedure the pathologist examined and collected tissue samples in \- 90°C. Using Westem blot method RCAS1 protein and β\-Actin control protein were analyzed. For the identification RCAS 1 anti\-RCAS 1 mouse Mo\-22\-1\-1 monoelonal antibody was used. Results RCAS1 was determined as a 32kDa band while β\-Actin control protein was identified as a 42 kDa band. The presence of RCAS 1 protein was identified in all cancerous tissue samples examined. The protein was also revealed in histological elear surgical margin and chronic inflammation of muco"]
