Search for: [Abstract = "s adjuvant containing Mycobacterium tuberculosis. Similar effects were observed after epicutaneous immunization with purified TLR ligands\: TLR2 \(peptidoglycan, lipoteichoic acid and zymosan A\), TLR3 \(dsRNA – polyI\:C\), TLR4 \(LPS\) and TLR9 \(ODN CpG\). Reversal of skin\-induced suppression by TLR ligands is dose dependant and the optimal working dose of TLR4 ligand \(LPS \) is 100 ng\/mouse. Based on the experiments described above, the most commonly used TLR4 ligand, LPS from E. coli, was used in subsequent experiments. Reversal of suppression was shown to not be strain dependent and can be induced independently on MHC haplotype, as was demonstrated by using mouse strains other than CBA\/J \(H\-2k\) e.g. BALB\/c \(H\-2d\) and C57Bl\/6 \(H\-2b\) Reversal of skin induced immunosupression is dependent on functional TLR4 receptor and the adaptor protein MyD88, as LPS did not overcome tolerance in C3H\/HeJ \(TLR4\-mutant mice\) and MyD88\-\/\- \(mice lacking an adaptor protein MyD88\) mice. Adoptive cell transfer experiments showed that reversal of suppression by LPS is via the induction of contrasuppressor cells in axillary and inginual lymph nodes. \; Negative selection experiments with monoclonal antibodies and rabbit complement revealed that these cells are TCRαβ\+ CD4\+ lymphocytes \(contasuppressor T cell, Tcs\). Experiments in an active immunization and adoptive transfer of CS employing three non\-crossreacti"]

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