Search for: [Abstract = "regulatory factors MYF5, MYOD, MYF6 and MYOG, as well as the expression of myotubes markers MYH3 and MYH2, in each protocol in different time points. Obtained cells were characterized by the presence of the extracellular markers CD10, CD24 and CD56 using flow cytometry. Cell fusion ability and vimentin presence with immunofluorescence were verified. Our results revealed that protocol III is the most efficient in obtaining myogenic cells. Furthermore, our results indicated that CD56 is not a specific marker for the evaluation of myogenic differentiation. Obtained results will be used in further studies investigating the role of SNAI1 in RMS."]

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